Identification of Genetic Regions Associated with Scrotal Hernias in a Commercial Swine Herd
نویسندگان
چکیده
In this paper, we have used two approaches to detect genetic associations with scrotal hernias in commercial pigs. Firstly, we have investigated the effects of runs of homozygosity (ROH) with the appearance of scrotal hernias, followed by a Genome Wide Association Study (GWAS). The phenotype classification was based on visual appearance of scrotal hernias. Each affected animal was matched to a healthy control from the same pen. In the total, 68 animals were genotyped using the Porcine SNP60 Beadchip, out of those, 41 animals had the presence of hernias and 27 were healthy animals. Fifteen animals were removed from the analysis due to differences in genetic background, leaving 18 healthy animals and 35 piglets with scrotal hernia. Further, the detection of extended haplotypes shared ROH were conducted for health (control) and affected (case) animals and a permutation test was used to test whether the ROH segments were more frequent in case/case pairs than non-case/case pairs. Using the ROH, we have identified an association (p = 0.019) on chromosome 2(SSC2) being segregated on animals with the presence of scrotal hernias. Using a GWAS, a region composed by 3 SNPs on the sexual chromosome X (SSCX) were associated with scrotal hernias (p < 1.6 × 10-5), this region harbors the Androgen Receptor Gene (AR).
منابع مشابه
Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism
OBJECTIVE This study was conducted to screen scrotal hernia in domesticated swine from selected breeders in the Philippines. This defect is associated with a cytosine to thymine mutation in the BCL-2 associated X protein (BAX) gene of swine. METHODS Genetic screening was done by DNA extraction followed by amplification and digestion using polymerase chain reaction-restriction fragment length ...
متن کاملAssociation between umbilical hernias and genetic line in a swine multiplication herd and methods to differentiate the role of sire in the incidence of umbilical hernias in offspring
Materials and methods: Gilt and boar progeny from 8276 litters of a genetic multiplier that used four dam lines and fi ve sire lines were observed for umbilical hernias by 11 weeks of age. Hernias were attributed to birth litter. Odds of umbilical hernia development were calculated using logistic regression and rates were calculated using Poisson regression. Negative binomial models using sire ...
متن کاملA genome-wide scan reveals candidate susceptibility loci for pig hernias in an intercross between White Duroc and Erhualian.
Pig scrotal/inguinal and umbilical hernias are the most prevalent congenital disorders in pigs and often cause animal welfare problems and economic loss. To identify susceptibility loci for these traits, a genome-wide scan with 194 microsatellite markers covering the pig genome was performed in a White Duroc x Erhualian resource population with 23 scrotal/inguinal F(2) animals, 50 umbilical F(2...
متن کاملMHC IIB Genetic Diversity and its Association With Humoral Immune Responses in Commercial Turkey
Background: Major histocompatibility complex (MHC) is one of the best characterized genetic regions controlling immune responses against vaccines. Identifying the association between MHC haplotypes and improved immune responses would be useful in genetic breeding strategies in animals. OBJECTIVES: MHC class II B genetic diversity and its association with humoral immune responses against Newcast...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 5 شماره
صفحات -
تاریخ انتشار 2018